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From 2013.igem.org

1>Prepare the 10% xylose solution and the 1mol/l nitrate solution, then filter.

2>Transform the biobrick into the competent cells, and culture them for 1h.

3>Prepare culture with different xylose concentrations, 1%, 0.1% and 0, and each concentration should be
prepared for two culture dishes, one for cam resistance and the other for kam resistance.

4>Prepare culture with different nitrate concentrations, 10m M, 2.5m M and 0.

Coat plates, and culture for one night.